Your favorite gene mutated in a human cell line.
The Human Gene Trap Mutant Collection is the largest collection of isogenic human cell lines that are deficient for the expression of single genes. The current collection is based on the human cell line KBM-7 , which is haploid for all chromosomes except chromosome 8 and a small part of chromosome 15. In these cells, genes are disrupted by the means of a retroviral gene trap . The collection currently comprises more than 9,400 clones, affecting more than 3,700 human genes annotated in RefSeq. A first draft of the collection has recently been published in Nature Methods . It is being expanded to cover the majority of expressed genes.
- Kotecki M, Reddy PS, Cochran BH. Isolation and characterization of a near-haploid human cell line. Exp Cell Res. 1999 Nov 1;252(2):273-80.
- Carette JE, Guimaraes CP, Varadarajan M, Park AS, Wuethrich I, Godarova A, Kotecki M, Cochran BH, Spooner E, Ploegh HL, Brummelkamp TR. Haploid genetic screens in human cells identify host factors used by pathogens. Science. 2009 Nov 27;326(5957):1231-5. doi: 10.1126/science.1178955.
- Bürckstümmer T, Banning C, Hainzl P, Schobesberger R, Kerzendorfer C, Pauler FM, Chen D, Them N, Schischlik F, Rebsamen M, Smida M, de la Cruz FF, Lapao A, Liszt M, Eizinger B, Guenzl PM, Blomen VA, Konopka T, Gapp B, Parapatics K, Maier B, Stöckl J, Fischl W, Salic S, Taba R, Knapp S, Bennett KL, Bock C, Colinge J, Kralovics R, Ammerer G, Casari G, Brummelkamp TR, Superti-Furga G and Nijman SMB. A reversible gene trap collection empowers haploid genetics in human cells. Nature Methods 10, 965–971 (2013) doi:10.1038/nmeth.2609